Informatics Publishing Limited

Baohui Men ¹, Chunkun Lin ¹, Zhifei Li ¹, Boyang Sun ¹

Affiliations
1 National Engineering Laboratory for Biomass Power Generation Equipment, Renewable Energy Institute, North China Electric Power University, Beijing-102206, CN

Abstract

The runoff data of Zhuba Station at Niqu river in water diversion area of Western Route Project (WRP) of South-North Water Transfers Project (SNWTP) from 1961 to 2010 were applied to estimate the coefficients of variation of hydrology, the peak pattern degree, and ample flow VS low flow, climate tendency rate and so on. The results were used to analyse the effects of hydrological regime on river discharge in the water supply area of the first stage project in WRP. The results demonstrated that: The annual river discharge is increasing in Niqu River which is the water supply area in the first stage project of WRP. The cumulative increased river discharge is 0.52×10⁸m³ within 50 years which is 2.6% larger than the average value. The runoff increased each year in non-flood season and decreased each year in flood season.

Keywords

Niqu River, Run-Off, Western Route Project, River Discharge Variation.

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Yun Xia ¹, Ermeng Yu ¹, Zhifei Li ¹, Deguang Yu ¹, Guangjun Wang ¹, Jun Xie ¹, Wangbao Gong ¹, Kai Zhang ¹

Affiliations
1 Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute of CAFS, Guangzhou, Guangdong 510380, CN

Abstract

To determine the composition of the bacterial communities associated with cyanobacteria in the freshwater ponds of Channa argus in intensive culture in the late culture period, we examined the bacterial community of non-cyanobacterial blooms freshwater ponds (C) and cyanobacterial blooms freshwater ponds (CB) using PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rDNA clone library. DGGE profiles showed that Microcystis sp. (JX391871.1), Leptospira sp. (JX067673.1) and Verrucomicrobium sp. (FN668203.2) existed only in CB, whereas Sphingomonas sp. (AB681542.1) and Paenibacillus sp. (JQ659563.1) were the C-specific bacteria. The 16S rDNA clone libraries of pond water showed that Actinobacteria (15 OTUs, 31.9%), Bacteroidetes (13 OTUs, 28.7%), Fibrobacteres (7 OTUs, 14.9%) and Proteobacteria (5 OTUs, 10.0%) were the dominant groups in C; the four dominant groups in CB were Bacteroidetes (17 OTUs, 34.7%), Actinobacteria (9 OTUs, 18.4%), Fibrobacteres (6 OTUs, 12.2%) and Proteobacteria (8 OTUs, 16.2%). In the clone library, distribution of sequences belonging to Fibrobacter in CB (22.2% of the 99 clones) was twice as that of C (11.1% of the 99 clones). In both DGGE map and 16S rDNA clone library the Firmicutes group (JQ659563.1 in DGGE and 3 OTUs in C library) existed only in C. These results suggested that the composition of bacterial communities changed with the cyanobacterial blooms in Channa argus intensive culture freshwater ponds, and whether the change of the Firmicutes-to-Bacteroidetes ratio could be used to predict cyanobacterial blooms in these types of ponds required further validation.

Keywords

PCR-DGGE, Clone Library, Cyanobacterial Blooms, Bacterial Community, Channa argus, Culture Ponds.

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